Header menu link for other important links
X
Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330
Published in
2013
PMID: 23519811
Volume: 69
   
Issue: 3
Pages: 313 - 315
Abstract
The NAD(P)H-dependent carbonyl reductase from Candida parapsilosis ATCC 7330 catalyses the asymmetric reduction of ethyl 4-phenyl-2-oxobutanoate to ethyl (R)-4-phenyl-2-hydroxybutanoate, a precursor of angiotensin-converting enzyme inhibitors such as Cilazapril and Benazepril. The carbonyl reductase was expressed in Escherichia coli and purified by GST-affinity and size-exclusion chromatography. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 1.86Å resolution. The asymmetric unit contained two molecules of carbonyl reductase, with a solvent content of 48%. The structure was solved by molecular replacement using cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae as a search model. © 2013 International Union of Crystallography All rights reserved.
About the journal
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
ISSN17443091
Open AccessNo
Concepts (25)
  •  related image
    Alcohol dehydrogenase
  •  related image
    CARBONYL REDUCTASE
  •  related image
    CINNAMYL ALCOHOL DEHYDROGENASE
  •  related image
    Fungal protein
  •  related image
    Recombinant protein
  •  related image
    Article
  •  related image
    Candida
  •  related image
    CANDIDA PARAPSILOSIS
  •  related image
    Chemistry
  •  related image
    Crystallization
  •  related image
    Enantioselectivity
  •  related image
    Enzymology
  •  related image
    Escherichia coli
  •  related image
    Genetics
  •  related image
    Saccharomyces cerevisiae
  •  related image
    STRUCTURAL HOMOLOGY
  •  related image
    X ray crystallography
  •  related image
    CANDIDA PARAPSILOSIS
  •  related image
    CARBONYL REDUCTASE
  •  related image
    Alcohol oxidoreductases
  •  related image
    Crystallography, x-ray
  •  related image
    Fungal proteins
  •  related image
    Recombinant proteins
  •  related image
    STRUCTURAL HOMOLOGY, PROTEIN
  •  related image
    CANDIDA PARAPSILOSIS