Characteristic impedance (Zc) of the blood vessel relates the pulsatile pressure to pulsatile blood flow velocity devoid of any wave reflections. Estimation of ZC is useful for indirect evaluation of local pulse wave velocity and crucial for solving wave separation analysis (WSA) which separates the forward-backward pressure and flow velocity waveforms. As opposed to conventional WSA, which requires simultaneous measurement of pressure and flow velocity waveform, simplified WSA relies on modelled flow velocity waveforms, mainly introduced for the aorta. This work uses a multi-Gaussian decomposition (MGD) modelled flow velocity waveform to estimate ZC by employing a frequency domain analysis, which is applicable to other arteries such as carotid. Thus obtained ZC is compared with Zc estimated from true flow velocity waveform for healthy (virtual) subjects taken for the carotid artery. The MGD modelled flow velocity waveform estimated ZC for a range of 4.98 to 34.79 with a group average of 16.43±0.10. The difference between the group average values of both ZC was only 4.72%. A statistically significant and strong correlation (r = 0.708, p < 0.0001) was observed for ZC obtained from MGD modelled flow velocity waveform with ZC obtained from actual flow velocity waveform. The bias for ZC1 between the two methods was 0.74, with confidence intervals (CIs) between 7.44 and -5.96 for the Bland-Altman analysis. Therefore, ZC from MGD modelled flow velocity waveform is a potential surrogate of the flow velocity model for WSA at the carotid artery. Clinical Relevance - This study provides a new method to derive characteristic impedance without the measurement of actual flow velocity waveform. The method requires a single pulse waveform (pressure or diameter) © 2022 IEEE.