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Enrichment of cardiomyocytes in primary cultures of murine neonatal hearts
Rajalakshmi Santhakumar, Prasanna Vidyasekar,
Published in
2015
PMID: 25836572
Volume: 1299
   
Pages: 17 - 25
Abstract
In vitro culture of neonatal murine cardiomyocytes is vital for understanding the functions of the heart. Cardiomyocyte cultures are difficult to maintain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step.
About the journal
JournalMethods in molecular biology (Clifton, N.J.)
ISSN19406029
Open AccessNo
Concepts (13)
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    Animal
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    Cell separation
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    Cytology
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    HEART MUSCLE CELL
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    Immunohistochemistry
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    Mouse
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    Newborn
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    Primary cell culture
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    Procedures
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    Animals
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    ANIMALS, NEWBORN
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    Mice
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    MYOCYTES, CARDIAC