A novel methodology to fabricate highly aligned array of equilateral gold NTs on ITO substrates was demonstrated. The ITO glass substrate was cleaned with a mild detergent solution, sonicated with deionized water and soaked in 10 % HCl solution for activation. They were then washed with water, dried and annealed at 450°C for 7 h and cooled in a desiccator. The glass slides were dipped in 30 mM APTMS solution in methanol for 1 h. Afterwards, they were washed with methanol and water in sequence and kept at 110°C for 2h. Seed coated substrate and an identical blank ITO glass plate were kept at 5 mm apart such that the conducting surfaces faced each other and were connected to the -ve and +ve terminals of a DC power supply. Contact mode AFM measurements were carried out using a reflux coated nanosensors silicon cantilever. It was observed that at higher potentials, irregular shapes are obtained.