The effect of calcination on P25 titania at different temperatures has been studied by XRD, nitrogen adsorption desorption isotherm, BET-surface area, pore volume, pore size, particle size and crystallite size in order to understand the conversion of anatase phase to rutile phase. The fraction of anatase and rutile has been determined using XRD. Although the crystallinity remains the same, the phase change of anatase to rutile is inevitable with temperature. The isotherms show the non porous characteristics of the P25 samples. In addition, nitrogen adsoption by the samples decreased appreciably with crystallinity. The pore volume and BET surface area show a large decrease at 900°C. The particle size, crystallite size, and pore size show an increasing trend with increase in temperature. Analysis of the physical characteristics indicates that P25 titania samples can be used for catalytic applications at less than 500°C.