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Development of a Next-Generation Fluorescent Turn-On Sensor to Simultaneously Detect and Detoxify Mercury in Living Samples
Abdul Malek, Kallol Bera, Shrutidhara Biswas, Govindaraj Perumal, Anand Kant Das, , ,
Published in American Chemical Society
2019
PMID: 30712342
Volume: 91
   
Issue: 5
Pages: 3533 - 3538
Abstract
Strategies for simultaneous detection and detoxification of Hg2+ using a single sensor from biological and environmental samples are limited and have not been realized in living organisms so far. We report a highly selective, small molecule "turn-on" fluorescent sensor, PYDMSA, based on the cationic dye Pyronin Y (PY) and chelating agent meso-2,3-dimercaptosuccinic acid (DMSA) for the simultaneous detection and detoxification of inorganic mercury (Hg2+). After Hg2+ detection, concomitant detoxification was carried out with sufficient efficacy in living samples, which makes the sensor unique. PYDMSA exhibits high selectivity for Hg2+ over other competing metal ions with an experimental detection limit of ∼300 pM in aqueous buffer solution. When PYDMSA reacts with Hg2+, the CS-C9 bond in the sensor gets cleaved. This results in the "turn-on" response of the fluorescence probe with a concomitant release of one equivalent of water-soluble Hg2+-DMSA complex which leads to a synchronous detoxifying effect. The sensor by itself is nontoxic to cells in culture and has been used to monitor the real-time uptake of Hg2+ in live cells and zebrafish larvae. Thus, PYDMSA is a unique sensor which can be used to detect and detoxify mercury at the same time in living samples. © 2019 American Chemical Society.
About the journal
JournalData powered by TypesetAnalytical Chemistry
PublisherData powered by TypesetAmerican Chemical Society
ISSN00032700
Open AccessNo
Authors (3)
Concepts (15)
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    Biology
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    Chelation
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    Detoxification
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    Dyes
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    Metal ions
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    Metals
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    AQUEOUS BUFFER SOLUTION
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    Environmental sample
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    Fluorescence probes
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    Fluorescent sensors
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    INORGANIC MERCURY
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    LIVING ORGANISMS
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    MESO-2 ,3-DIMERCAPTOSUCCINIC ACIDS
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    SIMULTANEOUS DETECTION
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    Fluorescence