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Determination of mimosine by a sensitive indirect spectrophotometric method
Kalyanaraman Lalitha
Published in Elsevier
2004
Volume: 63
   
Issue: 3
Pages: 635 - 640
Abstract
A simple and sensitive indirect spectrophotometric method is described for the determination of mimosine based on its reaction with diazotized sulfanilamide (DZSAM). DZSAM couples with N-(1-naphthyl)ethylenediamine (NEDA) forming a pink colored azodye, absorbing maximally at 540 nm (εmax=27 mM-1 cm-1). In the present method, mimosine was first reacted with known excess of DZSAM and the unreacted DZSAM was determined by coupling with NEDA. The reaction of mimosine with DZSAM proceeded optimally at neutral pH. The decrease in absorbance of the DZSAM-NEDA-coupled product obeyed Beer's law in the concentration range of 0.005-0.15 μg ml-1 of mimosine. The present method was applied to estimate mimosine in plant extracts containing lesser than 0.05 μg ml -1 with recovery at 99±0.41%. The method described is superior to other reported methods in terms of ease of adaptability and sensitivity. © 2003 Elsevier B.V. All rights reserved.
About the journal
JournalData powered by TypesetTalanta
PublisherData powered by TypesetElsevier
ISSN00399140
Open AccessNo
Concepts (24)
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    Absorption
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    Azo dyes
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    Concentration (process)
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    Optimization
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    Ph effects
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    Reaction kinetics
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    Sensitivity analysis
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    Spectrophotometry
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    CONCENTRATION RANGE
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    NEUTRAL PH
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    Organic chemicals
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    Amide
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    Azo compound
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    Dye
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    Ethylenediamine derivative
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    MIMOSINE
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    SULFANILIC ACID DERIVATIVE
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    Article
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    Chemical analysis
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    Chemical reaction
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    Color
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    Concentration (parameters)
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    Diazotization
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    pH