Bioimpedance Spectroscopy is a non-invasive, non-ionizing technique used for characterization of healthy and pathological tissues based on spectrum of their electrical properties. This can potentially be used as an adjunct diagnostic tool because spectral measurement provides much more clinically relevant information about the tissue than single frequency measurement. This paper presents a multifrequency bioimpedance measurement scheme to measure impedance varying from 1.6 Ω to 1.6 kΩ in the β dispersion region between 500 Hz and 1 MHz. In order to improve the accuracy of measurement, a three reference calibration algorithm based on quadratic Lagrange interpolation is employed which corrects the systematic errors mathematically. Preliminary experimental results show good agreement between calibrated values and standard values with a maximum relative magnitude error of 1.3 % and a maximum relative phase error of 0.6° over the entire frequency range. © 2016 IEEE.