To compare and assess silicone membrane-based sub-Tenon’s block (STB) simulator and animal eye model (goat’s eye) for practicing STB in terms of anatomical similarity and feel of texture of eye layers. The study included 34 participants (26 learners and 8 consultants) from tertiary ophthalmic centres. The participants were divided into groups A and B. Group A performed STB on the goat’s eyes before using the silicone membrane simulator. Group B performed STB on the simulator and further proceeded to the goat’s eye. Participants had to rate the anatomical similarity and feel of the texture for the simulator model on a scale of 0–10 and share their preference between the two models. In group A, the scores given to the simulator model and the feel of texture of layers were 8.05 ± 0.88 and 7.97 ± 1.07, respectively, and the scores given to the animal model and the feel of texture of layers were 8.11 ± 0.97 and 8.21 ± 0.88, respectively. Group B participants scored the simulator model and feel of texture of layers with 8.13 ± 0.95 and 8.25 ± 0.99, respectively. Overall, 89% participants preferred the simulator; the reasons included ease of usage, helpful warning system, absence of biological waste, and facility for repeatable training. The study validated anatomical accuracy, preference, and ability of usage of the STB simulator. For broader usage, further study involving higher number of participants is recommended. © 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.