In the present study, LIBS spectra of biomolecules have been analyzed. The basic biomolecules considered for the analysis were Carbohydrates, Protein and Nucleic acids. It was possible to detect the characteristic emission lines of C, H, N, O, S etc. in the above biomolecules. The samples were prepared for laser ablation as biofilms on glass slides. Interference from substrate was observed in the LIBS spectra of samples. Raman spectra would also be acquired in future studies to obtain molecular information for the biomolecules. © 2021, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.