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Biomimetic acellular detoxified glutaraldehyde cross-linked bovine pericardium for tissue engineering
Dillip Kumar Bishi, Soma Guhathakurta,
Published in
2013
PMID: 23827609
Volume: 33
   
Issue: 3
Pages: 1561 - 1572
Abstract

Glutaraldehyde (GLUT) processing, cellular antigens, calcium ions in circulation, and phospholipids present in the native tissue are predominantly responsible for calcification, degeneration, and lack of natural microenvironment for host progenitor cell migration in tissue implants. The study presents an improved methodology for adhesion and proliferation of endothelial progenitor cells (EPCs) without significant changes in biomechanical and biodegradation properties of the processed acellular bovine pericardium. The anti-calcification potential of the processed tissue was enhanced by detoxification of GLUT-cross-linked bovine pericardium by decellularization, pretreating it with ethanol or removing the free aldehydes by citric acid treatment and lyophilization. The treated tissues were assessed for biomechanical properties, GLUT ligand quantification, adhesion, proliferation of EPCs, and biodegradability. The results indicate that there was no significant change in biomechanical properties and biodegradability when enzymatic hydrolysis (p > 0.05) is employed in detoxified acellular GLUT cross-linked tissue (DBP-G-CA-ET), compared with the native detoxified GLUT cross-linked bovine pericardium (NBP-G-CA-ET). DBP-G-CA-ET exhibited a significant (p > 0.05) increase in the viability of EPCs and cell adhesion as compared to acellular GLUT cross-linked bovine pericardium (p < 0.05). Lyophilized acellular detoxified GLUT cross-linked bovine pericardium, employed in our study as an alternative to conventional GLUT cross-linked bovine pericardium, might provide longer durability and better biocompatibility, and reduce calcification. The developed bovine pericardium patches could be used in cardiac reconstruction and repair, arteriotomy, soft tissue repair, and general surgical procedures with tissue regeneration dimensions. © 2012 Elsevier B.V. All Rights Reserved.

About the journal
JournalMaterials Science and Engineering C
ISSN09284931
Open AccessNo
Concepts (90)
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    ACELLULAR BOVINE PERICARDIUM
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    Acid treatments
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    BIOMECHANICAL PROPERTIES
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    BOVINE PERICARDIUM
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    CALCIUM IONS
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    CARDIAC RECONSTRUCTION
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    DECELLULARIZATION
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    Endothelial progenitor cells
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    GLUTARALDEHYDES
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    Microenvironments
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    PROGENITOR CELL
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    Soft tissue
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    Surgical procedures
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    TISSUE IMPLANTS
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    Tissue regeneration
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    Aldehydes
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    Biocompatibility
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    Biodegradation
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    Biomechanics
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    Biomimetics
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    Biomineralization
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    Bone
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    Cell adhesion
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    Citric acid
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    Crosslinking
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    Detoxification
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    Enzymatic hydrolysis
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    Ethanol
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    Mammals
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    Phospholipids
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    Tissue engineering
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    Tissue
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    Biomimetic material
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    CROSS LINKING REAGENT
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    Glutaraldehyde
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    CROSS LINKING REAGENT
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    Animal
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    Bovinae
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    Cell death
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    Cell proliferation
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    Cell shape
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    Cytology
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    Drug effects
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    Endothelium cell
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    Immunohistochemistry
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    Metabolism
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    PERICARDIUM
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    Phenotype
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    Physiology
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    Procedures
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    Sheep
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    Stem cell
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    Ultrastructure
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    Article
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    BOVINE PERICARDIUM
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    Cattle
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    Cross-linking
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    DBP-G
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    DBP-G-CA
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    DBP-G-CA-ET
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    DECELLULARIZATION
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    DECELLULARIZED BOVINE PERICARDIA-GLUTARALDEHYDE
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    DECELLULARIZED BOVINE PERICARDIA-GLUTARALDEHYDE-CITRIC ACID
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    DECELLULARIZED BOVINE PERICARDIA-GLUTARALDEHYDE-CITRIC ACID-ETHANOL
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    Drug effect
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    Endothelial progenitor cell
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    Methodology
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    NATIVE BOVINE PERICARDIA-GLUTARALDEHYDE-CITRIC ACID-ETHANOL
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    NBP-G-CA-ET
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    PERICARDIUM
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    Animals
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    Biomechanical phenomena
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    Biomimetic materials
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    Cross-linking reagents
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    Endothelial cells
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    GLUTARAL
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    PERICARDIUM
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    Stem cells
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    BOVINE PERICARDIUM
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    DBP-G
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    DBP-G-CA
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    DBP-G-CA-ET
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    DECELLULARIZATION
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    DECELLULARIZED BOVINE PERICARDIA-GLUTARALDEHYDE
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    DECELLULARIZED BOVINE PERICARDIA-GLUTARALDEHYDE-CITRIC ACID
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    DECELLULARIZED BOVINE PERICARDIA-GLUTARALDEHYDE-CITRIC ACID-ETHANOL
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    NATIVE BOVINE PERICARDIA-GLUTARALDEHYDE-CITRIC ACID-ETHANOL
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    NBP-G-CA-ET
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    GLUTARAL
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    PERICARDIUM