A novel biosensor for dopamine (DA) detection was fabricated using TiO 2 nanotube and Au/TiO2 nanotube films on glassy carbon electrode (GCE). The Au/TiO2 nanotubes on electrode showed better electrocatalytic activity towards the detection of DA which was attributed to its excellent electron conductive network. The biosensor elicited sensitivity of 22 nA/μM with a linearity of detection localized in the concentration range from 5120 μM with correlation coefficient of 0.99. The detection limit of DA for the Au/TiO2 nanotube biosensor was found to be ∼3 μM (S/N = 3). In addition, the fabricated sensor showed good anti-interference capability towards biological compounds such as ascorbic acid and uric acid. In conclusion, Au/TiO2 nanotube biosensor exhibits excellent catalytic activity, selectivity and simplicity for the detection of dopamine. © 2011 World Scientific Publishing Company.