In this work, an attempt has been made to analyze the preterm (gestation period ≤ 37 weeks) condition using uterine electromyography (EMG) signals and multifractal detrended fluctuation analysis (MFDFA). The signals recorded from the electrodes placed on the surface of abdomen are used for this study and these are obtained from a publically available online database. These signals are preprocessed using 4-pole digital Butterworth filter. The preprocessed signals are subjected to MFDFA to extract multifractal features namely maximum singularity exponent, peak singularity exponent, strength of multifractality and exponent index. Generalized Hurst exponent extracted from the signals indicate that uterine EMG signals show multifractal behavior in preterm condition. Among the extracted features the coefficient of variation is found to be lower for peak singularity exponent. This indicates that this feature have lower inter-subject variability. Hence, it appears that the multifractal features can help in the assessment of uterine EMG signals for preterm detection. © 2018 IEEE.