The regeneration of Saccharomyces cerevisiae, NCIM 3288, cells from its sphaeroplasts were found to be influenced by a number of factors. The most suitable conditions of regeneration were also dependent on growth medium, that is, using malt-extractglucose-yeast extract-peptone (MGYP) medium: mannitol 0.7 M, pH 6.5, 30 °C and using yeast extract-peptone-glucose (YPG) medium: sucrose 0.7 M, pH 5.0 and 30 °C. The maximum regeneration frequency was observed in YPG medium. © 1994 Springer-Verlag.

T Panda

Department Of Chemical Engineering

J. Anjani Kumari

article

nonhuman

priority journal

Regeneration

Saccharomyces cerevisiae

spheroplast

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Analysis of factors responsible for the regeneration to intact cells from sphaeroplasts of Saccharomyces cerevisiae

Bioprocess Engineering

An attempt to produce ethanol from a cellulosic substrate by a single-stage process using intergeneric hybrids obtained from Trichoderma reesei QM 9414 and Saccharomyces cerevisiae NCIM 3288 fusants is described. Two hundred one fused strains were isolated using two different combinations of hybridization, followed by polyethylene glycol treatment of protoplasts derived from parent and mutant strains. Some fusants were either diploids or heterokaryons, as verified by a protoplast fused system obtained by auxotrophic mutants derived from T. reesei QM 9414 and S. cerevisiae NCIM 3288. Of the 201 fusants, 170 were found to be relatively stable. Two fusants (M14 and M62) showed the highest synthesis of ethanol from filter paper cellulose, producing 0.0149 and 0.0191 g l-1, respectively. © 1994.

Enzyme and Microbial Technology

Intergeneric hybridization of Trichoderma reesei QM9414 and Saccharomyces cerevisiae NCIM 3288 by protoplast fusion

Efficient synthesis of large numbers of viable sphaeroplast from Saccharomyces cerevisiae has been found to be influenced by a number of factors. In this case, Trichoderma harzianum, NCIM 1185, culture filtrate has been used to prepare sphaeroplast from Saccharomyces cerevisiae, NCIM 3288. A method has been devised to isolate large number of viable sphaeroplast from the cell. Detailed analysis of various factors affecting the formation of sphaeroplasts from Saccharomyces cerevisiae has not yet been reported. This study showed critical analysis of various factors which influenced sphaeroplast formation. Most suitable conditions were: Age of the organism in slant - 1 d, cell age in liquid medium - 24 h, time of incubation of cell with 0.3% β-mercaptoethanol - 30 min, level of lytic ezyme concentration - 79.2 ml, concentration of cell (dry wt. equivalent) - 0.1262 g, time of contact with lytic enzyme - 25 min, temperature of sphaeroplast formation - 30 °C, phosphate buffer - 25 mM of pH 6.5 and KCl as osmotic stabilizer - 0.7 M. © 1992 Springer-Verlag.

Critical analysis of factors influencing sphaeroplast generation from Saccharomyces cerevisiae

Efficient synthesis of a large number of viable protoplasts from Trichoderma longibrachiatum (Trichoderma reesei) mycelium has been found to be influenced by a number of factors. In this case, Trichoderma harzianum NCIM 1185 culture filtrate has been used to prepare protoplasts from Trichoderma longibrachiatum QM 9414. A method has been devised to isolate a large number of viable protoplasts from the mycelium. Detailed analysis of different factors affecting the synthesis of protoplasts from T. longibrachiatum has not yet been reported. The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml; mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzymes, 2 h; temperature of protoplasting, 30°C; phosphate buffer, 25 mm, pH 6.5; KCl as osmotic stabilizer, 0.7 m. Proper spreading of the mycelium in contact with the lytic enzymes was also found to be necessary. © 1992.

Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium

Yeast Cell Envelopes: Biochemistry, Biophysics, and Ultrastructure

Morphogenesis in Protoplasts

Makers of bioprocess engineering

Current Microbiology

Yeast protoplasts immobilized in alginate: Cell wall regeneration and reversion to cells

Journal	Data powered by TypesetBioprocess Engineering
Publisher	Data powered by TypesetSpringer-Verlag
ISSN	0178515X
Open Access	No