ImageQuant Analyzer is an affordable, compact lateral flow immunoassay (LFIA) based PoC diagnostic reader capable of fast quantitative evaluation of analyte concentration from a sample. In this paper, ImageQuant's evolved hardware and software algorithm is described. To validate improvements, TSH analyte from human sera samples were analyzed. Correlation plots between proposed measurements and a Gold Standard reference show superiority of our system (R = 0.96) in predicting TSH concentrations. Validation results for test trials within a single instrument and across instruments (intra-variability < 2 % and inter-variability < 5.5 %) also signify the merits of the newly improved algorithm. © 2018 IEEE.

Mohanasankar Sivaprakasam

Department of Electrical Engineering

Jayaraj Joseph

Malay Ilesh Shah

immunology

DIAGNOSTIC INSTRUMENTATION

IMAGEQUANT

immunoassay

LFIA

POINT-OF-CARE TESTING

image enhancement

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

An Improved System for Quantitative Immunoassay Measurement in ImageQuant

Proceedings of IEEE Sensors

In the emergency diagnosis of patients, acute myocardial infarction (AMI) is always time-consuming to diagnose, and the process requires multiple laboratory procedures, expensive equipment and skilled workers. Herein, we developed an easy-to-use, low-cost and portable fluorescent lateral flow immunoassay based on paper microfluidics for the point-of-care diagnostics of non-communicable diseases. The fluorescent lateral flow immunoassay can produce results in less than 10 minutes, and the limit of detection (LOD) is 0.019 ng ml-1. The slope was linear from 0 to 100 ng ml-1; the equation is y = 0.0342e2.1181x and R2 = 0.9618, which are distinctive features that ensure maximum amplification of the signal and recording of quantitative values by an analyser. The detection sensitivity showed an exceptional increase to 0.01 ng ml-1. Compared with conventional bioassay readers, our analyser shows some advantages to easily, clearly and effectively read data. The present point-of-care test for cardiac troponin I decreases the turnaround time and has a high coefficient of variation even at lower concentrations of troponin. So, the development of lateral flow assay-based point-of-care assays with higher analytical performance for real world samples can decrease the rule-out time for AMI in emergency departments and other fields. © 2019 The Royal Society of Chemistry.

Fulltext

Analyst

A paper microfluidics-based fluorescent lateral flow immunoassay for point-of-care diagnostics of non-communicable diseases

Lateral flow immunoassays (LFIA) used in rapid quantitative point of care testing require an accurate, reliable and easy to operate instrument to read the LFIA kit and calculate the quantitative result value. We present iQuant® Analyser, an immunoassay reader designed for reading the Quanti® range of LFIA test kits for key markers such as HbA1C, Vitamin D, TSH etc. The instrument utilizes a laser based confocal optics system to capture the test and control lines from the LFIA kit, digitizes the fluorescent signal with high spatiotemporal resolution, computes necessary peak area ratios, applies calibration curves and declares the final result in an automated manner with minimal operator input. The instrument uses kit specific calibration information embedded on each LFIA test kit, to compute the final clinical parameter without using any external calibration chip. An intuitive icon based interface enables easy operation with minimal key presses, suited for point of care applications. The technology is designed in a modular manner to enable the instrument to perform tests on various parameters such as HbA1C, TSH, and Vitamin D etc without any hardware changes, using test-specific LFIA kits. The functional performance of the iQuant Analyser was verified over the range of expected area ratio values with standard reference cartridges that provided stable fluorescent lines. Repeatability of the instrument was found to be excellent with coefficient of variation (CoV) of area ratios found to be less than 1%. The inter-instrument reproducibility was also found to be good with CoV less than 4 %. Tests using blood samples with Quanti LFIA kits verified the accuracy of HbA1C results to be acceptable as per international standards with errors < 4 %. The iQuant Analyser is a portable, easy to use rapid quantitative immunoassay reader best suited for point of care applications. © 2017 IEEE.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

IQuant™ Analyser: A rapid quantitative immunoassay reader

Electroanalysis

Lateral Flow Immunoassays - from Paper Strip to Smartphone Technology

Annals of Pediatric Endocrinology & Metabolism

Iodine and thyroid function

Biosensors and Bioelectronics

Cell-phone-based measurement of TSH using Mie scatter optimized lateral flow assays

Lab on a Chip

Integrated rapid-diagnostic-test reader platform on a cellphone

Journal	Data powered by TypesetProceedings of IEEE Sensors
Publisher	Data powered by TypesetInstitute of Electrical and Electronics Engineers Inc.
ISSN	19300395
Open Access	No