ImageQuant Analyzer is an affordable, compact lateral flow immunoassay (LFIA) based PoC diagnostic reader capable of fast quantitative evaluation of analyte concentration from a sample. In this paper, ImageQuant's evolved hardware and software algorithm is described. To validate improvements, TSH analyte from human sera samples were analyzed. Correlation plots between proposed measurements and a Gold Standard reference show superiority of our system (R = 0.96) in predicting TSH concentrations. Validation results for test trials within a single instrument and across instruments (intra-variability < 2 % and inter-variability < 5.5 %) also signify the merits of the newly improved algorithm. © 2018 IEEE.