The MoS2 2D nanosheets prepared by the hydrothermal method are characterized for its structural and morphological features. The structural studies show the pure form of MoS2 with hexagonal phase. The morphological study done with Tunnelling Electron Microscope shows that there are 6 to 10 2D atomic layers of MoS2. The sensitivity of this 2D MoS2towards Sodium ion was done using Cyclic Voltammetry. The results confirm that 2D MoS2modified Carbon electrodes are good candidates for selective sensing of sodium ion in sweat. The sensing mechanism is explained. © 2020 American Institute of Physics Inc.. All rights reserved.